Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
(i) Have origin of replication(Ori) (ii) a selectable marker (iii) at least one recognition site.
1 Answers 3 viewsThe experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which...
1 Answers 1 viewsIf the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...
1 Answers 1 viewsi. Restriction endonucleases: for cutting the desired DNA at desired places ii. Gel electrophoresis: for separating the desired DNA fragments iii. Ligase enzyme: for creating recombinant DNA molecule. iv. DNA delivery system: like...
1 Answers 2 viewsThe experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides...
1 Answers 1 viewsIf the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...
1 Answers 1 viewsIn the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...
1 Answers 1 viewsGel electrophoresis , Since DNA fragments are negatively charged , they move towards anode (under an electric field ) through a medium / matrix / agarose gel , The fragments...
1 Answers 1 viewsWashing clothes, cleaning, sweeping, cooking etc.
1 Answers 1 viewsa. Formation of phosphodiester bond between two DNA fragments
1 Answers 1 views