In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells.

Monjur Alahi

In recombinant DNA technology, vectors are used to transfer a gene of
interest in the host cells. Mention any three features of vectors that are most
suitable for this purpose.


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Salim Ahmed Kawsar

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(i) Have origin of replication(Ori)
(ii) a selectable marker
(iii) at least one recognition site.

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Salim Ahmed Kawsar

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(i) Have origin of replication(Ori) 

(ii) Have a selectable marker 

(iii) Have at least one recognition site.

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An extra chromosomal segment of circular DNA is used to carry gene of interest into the host cell. What is the name given to it ?

Answer is: Plasmid

1 Answers 1 views
You have created a recombinant DNA molecule by ligating a gene to a plasmid vector

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which...

1 Answers 1 views
You have identified a useful gene in a bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.

After identifying a useful gene in bacteria, following steps should be undertaken (1) Isolation of useful gene using Restriction Endonucleases                      ↓ (2) Transferring...

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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

i. Restriction endonucleases: for cutting the desired DNA at desired places ii. Gel electrophoresis: for separating the desired DNA fragments iii. Ligase enzyme: for creating recombinant DNA molecule. iv. DNA delivery system: like...

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You have created a recombinant DNA molecule by ligating a gene to a plasmid vector.

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides...

1 Answers 1 views
What would happen when you grow a recombinant in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing.

In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...

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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool. 1 Answers 1 views
Name and explain the technique that heips in the separation of DNA fragments for DNA recombinant technology experiments.

Gel electrophoresis , Since DNA fragments are negatively charged , they move towards anode (under an electric field ) through a medium / matrix / agarose gel , The fragments...

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The role of DNA ligase in the construction of a recombinant DNA molecule is:

a. Formation of phosphodiester bond between two DNA fragments

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A bacterial cell was transformed with a recombinant DNA that was generated using a human gene.

a. Human gene may have intron which bacteria cannot process

1 Answers 1 views
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