You have created a recombinant DNA molecule by ligating a gene to a plasmid vector.

(i) Have origin of replication(Ori) (ii) a selectable marker (iii) at least one recognition site.

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which...

In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced...

In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced...

In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...

(a) : vector a = 8vector b vector c = - 7vector b vector a and vector b are parallel and vector b and vector c are antiparallel. Thus vector a and vector c are...

(b) It is possible to have | vector C | < | vector A | and | vector C | < | vector B | 

a. Formation of phosphodiester bond between two DNA fragments

a. Human gene may have intron which bacteria cannot process

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