The role of DNA ligase in the construction of a recombinant DNA molecule is:

(i) Have origin of replication(Ori) (ii) a selectable marker (iii) at least one recognition site.

(i) helicase – opens the helix (ii) topoisomerases – removes the supercoiling of DNA (iii) Primase: synthesises RNA primer (iv) Telomerase: to synthesises the DNA of telomeric end of chromosomes.

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which...

If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...

i. Restriction endonucleases: for cutting the desired DNA at desired places ii. Gel electrophoresis: for separating the desired DNA fragments iii. Ligase enzyme: for creating recombinant DNA molecule. iv. DNA delivery system: like...

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides...

If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...

In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...

Gel electrophoresis , Since DNA fragments are negatively charged , they move towards anode (under an electric field ) through a medium / matrix / agarose gel , The fragments...

d. the absence of the following: E.coli