You have created a recombinant DNA molecule by ligating a gene to a plasmid vector

(i) Have origin of replication(Ori) (ii) a selectable marker (iii) at least one recognition site.

In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced...

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides...

In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced...

In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...

(a) : vector a = 8vector b vector c = - 7vector b vector a and vector b are parallel and vector b and vector c are antiparallel. Thus vector a and vector c are...

(b) It is possible to have | vector C | < | vector A | and | vector C | < | vector B | 

a. Formation of phosphodiester bond between two DNA fragments

a. Human gene may have intron which bacteria cannot process

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