You have chosen a plasmid as vector for cloning your gene. However this vector plasmid lacks a selectable marker. How would it affect your experiment?

Monjur Alahi

You have chosen a plasmid as vector for cloning your gene. However this vector plasmid lacks a selectable marker. How would it affect your experiment?


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Salim Ahmed Kawsar

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In a gene cloning experiment, first a recombinant DNA molecule is constructed, where the gene of interest is ligated to the vector, [The step would not be affected] and introduced inside the host cell (transformation). Since, not all the cells get transformed with the recombinant/plasmid DNA, in the absence of selectable marker, it will be difficult to distinguish between transformants and non-transformant, because role of selectable marker is in the selection of transformants.

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