Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

Monjur Alahi

Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.


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Salim Ahmed Kawsar

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i. Restriction endonucleases: for cutting the desired DNA at desired places
ii. Gel electrophoresis: for separating the desired DNA fragments
iii. Ligase enzyme: for creating recombinant DNA molecule.
iv. DNA delivery system: like electroporation, microinjection, gene gun method.
v. Competant host (usually bacteria / yeast): to take up recombinant DNA.

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Salim Ahmed Kawsar

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i. Restriction endonucleases: for cutting the desired DNA at desired places

 ii. Gel electrophoresis: for separating the desired DNA fragments

 iii. Ligase enzyme: for creating recombinant DNA molecule.

 iv. DNA delivery system: like electroporation, microinjection, gene gun method.

 v. Competant host (usually bacteria / yeast): to take up recombinant DNA

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Salim Ahmed Kawsar

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a. Restriction endonucleases: for cutting the desired DNA at desired places

b. Gel electrophoresis: for separating the desired DNA fragments

c. Ligase enzyme: for creating recombinant DNA molecule. 

d. DNA delivery system: like electroporation, microinjection, gene gun method.

e. Competent host (usually bacteria/yeast): to take up recombinan

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In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells.

(i) Have origin of replication(Ori) (ii) a selectable marker (iii) at least one recognition site.

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You have created a recombinant DNA molecule by ligating a gene to a plasmid vector

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which...

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The restriction enzymes that are used in construction of recombinant DNA molecule are endonuclease

If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...

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You have created a recombinant DNA molecule by ligating a gene to a plasmid vector.

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides...

1 Answers 1 views
The restriction enzymes that are used in construction of recombinant DNA molecule are endonucleases

If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...

1 Answers 1 views
What would happen when you grow a recombinant in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing.

In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...

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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool. 1 Answers 1 views
Name and explain the technique that heips in the separation of DNA fragments for DNA recombinant technology experiments.

Gel electrophoresis , Since DNA fragments are negatively charged , they move towards anode (under an electric field ) through a medium / matrix / agarose gel , The fragments...

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Washing clothes, cleaning, sweeping, cooking etc.

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The role of DNA ligase in the construction of a recombinant DNA molecule is:

a. Formation of phosphodiester bond between two DNA fragments

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