Name and explain the technique that heips in the separation of DNA fragments for DNA recombinant technology experiments.

Monjur Alahi

Name and explain the technique that heips in the separation of DNA fragments for DNA recombinant technology experiments. How can these separated DNA fragments be visualised?


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Salim Ahmed Kawsar

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Gel electrophoresis , Since DNA fragments are negatively charged , they move towards anode (under an electric field ) through a medium / matrix / agarose gel , The fragments separate (resolve) according to their size through sieving effect provided by agarose gel , The separated DNA fragments can be visualised after staining the DNA with ethidium bromide , followed by exposure to UV radiation

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