In the given process of separation and isolation of DNA fragments, some of the steps are missing, Complete the missing steps :

Monjur Alahi

In the given process of separation and isolation of DNA fragments, some of the steps are missing, Complete the missing steps :
A : Restriction digestion of DNA fragments

B : . ...........................................
C : Staining with ethidium bromide

D : Visualisation in U.V. light

E : . ...........................................
F : Purification of DNA fragments


0 like 0 dislike
1 views
Share with your friends

1 Answers

Salim Ahmed Kawsar

Call

B-Gel Electrophoresis
E-Elution

0 like 0 dislike
1 views
Talk Doctor Online in Bissoy App
The best and latest technique for isolation, purification and separation of organic compounds is:

Chromatography is the most useful and the latest technique of separation and purification of organic compounds. It was first used to separate a mixture of coloured substances. 

1 Answers 1 views
Which separation techniques will apply for the separation of the following? (a) Sodium chloride from its solution in water

(a) Crystallization or Evaporation.  (b) Sublimation.  (c) Centrifugation or Sedimentation.  (d) Chromatography.  (e) Centrifugation.  (f) Separating funnel.  (g) Hand-picking.  (h) Magnetic separation.  (i) Winnowing.  (j) Centrifugation.

1 Answers 1 views
Given below is a sequence of steps in the processing of wool. Which are the missing steps?

Shearing, scouring, sorting, fibre spinning, fibre drying, rolling in silk yarn.

1 Answers 1 views
Name and describe the technique that helps in separation and isolation of DNA fragments for biotechnology experiment.

Gel - electrophoresis , cutting of DNA by restriction endonucleases results in fragments of DNA , DNA fragments are negatively charged molecules, they can be separated by forcing them to...

1 Answers 1 views
Name and explain the technique that heips in the separation of DNA fragments for DNA recombinant technology experiments.

Gel electrophoresis , Since DNA fragments are negatively charged , they move towards anode (under an electric field ) through a medium / matrix / agarose gel , The fragments...

1 Answers 1 views
Name the enzyme that helps to join DNA fragments.

(DNA) ligase

1 Answers 1 views
The DNA fragments separated on an agarose gel can be visualised after staining with :

The correct answer is (3) Ethidium bromide (Et Br) is used to stain the DNA fragments and will appear as orange coloured bands when kept under UV light.

1 Answers 1 views
DNA fragments are: (1) Negatively charged

The correct answer is (1) DNA fragments are negatively charged because of presence of phosphate group.

1 Answers 1 views
During DNA replication, Okazaki fragments are used to elongate:

The correct answer is (3) Two DNA polymerase molecules simultaneously work at the DNA fork, one on the leading strand and the other on the lagging strand. DNA polymerase synthesizes each...

1 Answers 1 views
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

The correct answer is (1) DNA fragments during gel electrophoresis, separate (resolve) according to their size due to sieving effect provided by agarose gel

1 Answers 1 views
X