The DNA fragments separated on an agarose gel can be visualised after staining with :
(1) Acetocarmine
(2) Aniline blue
(3) Ethidium bromide
(4) Bromophenol blue
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultra Violet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide...
The reasons are as follows:
(i) DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded.
(ii) Electrodes were put in opposite...
A compound called Ethidium Bromide stains DNA, which on irradiating with UltraViolet, fluoresce and gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide...
The reasons are as follows:
(i) DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded.
(ii) Electrodes were put in opposite...
[Hint :
(a) For a sol : Dispersed phase : Solid; Dispersion medium :Liquid For a gel : Dispersed phase : Liquid, Dispersion medium : Solid
(b) For a gel : Dispersed phase : Liquid, Dispersion medium Solid
For an emulsion : Dispersed...
Removing poverty and building a modern technical and industrial base were important objectives for the new nation. The Planning Commission was set up in 1950 to plan and execute policies...
The correct answer is (1)
DNA fragments during gel electrophoresis, separate (resolve) according to their size due to sieving effect provided by agarose gel
