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The translated sequence can then be compared to a database of protein sequences with the hope of finding a strong similarity to a protein of known function, and hence to identify the function of the cloned EST.

The translated sequence can then be compared to a database of protein sequences with the hope of finding a strong similarity to a protein of known function, and hence to identify the function of the cloned EST. Correct Answer True

The translated sequence can then be compared in the mentioned way hence to identify the function of the cloned EST. The corresponding cDNA clone of the gene of interest can then be obtained and the gene completely sequenced.

Related Questions

Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm?
P. A larger E-value indicates higher sequence similarity.
Q. E-value -10 indicates sequence homology.
R. A higher BLAST score indicates higher sequence similarity.
S. E-value > 10-10 indicates sequence homology.
If the two sequences share significant similarity, it is extremely ______ that the extensive similarity between the two sequences has been acquired randomly, meaning that the two sequences must have derived from a common evolutionary origin.
TBLASTX queries protein sequences to anucleotide sequence database with the sequences translated in all six reading frames.
Sequence similarity and sequence identity are synonymous for nucleotide sequences and protein sequences as well.
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Annotation is based on finding significant alignment to sequences of known function in database similarity searches.
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