Sequencing cDNA Libraries of Expressed Genes, Submission of Sequences to the Databases MCQ

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• Granting agency requires a certain level of accuracy in case of errors. Which of the given statements is untrue regarding it?
  ক

  In other laboratories, such as those performing a single-attempt sequencing of ESTs, the error rate may be much higher, approximately 1 in 100, including incorrectly identified bases and inserted or deleted bases

  খ

  Incorrect bases always translate to the right amino acid

  গ

  Base insertions/deletions will cause frame-shifts in the sequence

  ঘ

  Making alignment with a protein sequence becomes difficult because of frameshifts
• Which of the given statements is untrue?
  ক

  There is no detailed check of sequence accuracy prior to submission to GenBank and other databases

  খ

  Often, a sequence is submitted at the time of publication of the sequence in a journal article, providing a certain level of checking by the editorial peer review process

  গ

  No sequence is submitted without being published or prior to publication

  ঘ

  In laboratories performing large sequencing projects, such as those engaged in the Human Genome Project or the genome projects of model organisms, the granting agency requires a certain level of accuracy of the order of 1 possible error per 10 kb
• Which of the given statements is incorrect?
  ক

  The simplest and newest way of submitting sequences is through the Web site on a Web form page called BankIt

  খ

  The sequence can also be annotated with information about the sequence, such as mRNA start and coding regions

  গ

  The submitted form is transformed into GenBank format and returned to the submitter for review before being added to GenBank

  ঘ

  Sequin does not run on UNIX
• NCBI reviews new entries and updates existing ones, as requested.
  ক

  True

  খ

  False
• Investigators are encouraged to submit their newly obtained sequences directly to a member of the International Nucleotide Sequence Database Collaboration, such as the NCBI, DDBJ, and EMBL.
  ক

  True

  খ

  False
• The translated sequence can then be compared to a database of protein sequences with the hope of finding a strong similarity to a protein of known function, and hence to identify the function of the cloned EST.
  ক

  True

  খ

  False
• An important development for computational purposes was the decision by Craig Venter to prepare databases of partial sequences of the expressed genes, called expressed sequence tags or ESTs.
  ক

  True

  খ

  False
• Using cDNA sequence with the __________ it is much simpler to locate protein-encoding sequences in these molecules.
  ক

  exons taken out

  খ

  exons removed

  গ

  introns added

  ঘ

  introns removed
• cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).
  ক

  True

  খ

  False
• Two common goals in sequence analysis are to identify sequences that encode proteins, which determine all cellular metabolisms, and to discover sequences that regulate the expression of genes or other cellular processes.
  ক

  True

  খ

  False