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In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells.
(i) Have origin of replication(Ori) (ii) a selectable marker (iii) at least one recognition site.
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For producing a recombinant protein (for therapeutic purpose) in large scale, which vector would you choose
High-copy-number, because higher the copy number of vector plasmid, higher the copy number of gene and consequently, the protein coded by the gene is produced in high amount.
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A wine maker and a molecular biologist who has developed a recombinant vaccine, both claim themselves to be biotechnologist.
Both. As biotechnology is a very wide area which deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organism to produce products and...
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You have created a recombinant DNA molecule by ligating a gene to a plasmid vector
The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which...
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The restriction enzymes that are used in construction of recombinant DNA molecule are endonuclease
If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...
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What would happen when you grow a recombinant in a bioreactor but forget to add antibiotic
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...
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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
i. Restriction endonucleases: for cutting the desired DNA at desired places ii. Gel electrophoresis: for separating the desired DNA fragments iii. Ligase enzyme: for creating recombinant DNA molecule. iv. DNA delivery system: like...
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A wine maker and a molecular biologist who has developed a recombinant vaccine
Both. As biotechnology is a very wide area which deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organism to produce products and...
1 Answers 1 views
You have created a recombinant DNA molecule by ligating a gene to a plasmid vector.
The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides...
1 Answers 1 views
What would happen when you grow a recombinant in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing.
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...
1 Answers 1 views