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How many primers are used in the process of reverse transcriptase amplification?

How many primers are used in the process of reverse transcriptase amplification? Correct Answer 2

The first primer of oligo – T is used during the reverse transcription of the mRNA to cDNA. The cDNA produced is a single stranded molecule thus synthesis of its complementary strand is necessary. A poly G/C tail is added to its 3’ end and thus the second primer is used against this poly G/C tail for the synthesis of the cDNA duplex.

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