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Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'

Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3' Correct Answer P and S

Related Questions

Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
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4. Replication takes place bidirectionally
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2. A DNA chain is initiated on the 3'- OH end of the primer
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4. Okazaki fragments are joined by endonuclease
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P. Mg2+ concentration
Q. pH
R. Annealing temperature