A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg2+. Which one of the following is missing in the reaction mixture?

A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg2+. Which one of the following is missing in the reaction mixture? Correct Answer Primers

Bissoy MCQ

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction?
The Taq DNA polymerase is DNA polymerase _________ enzyme from the bacterium Thermus aquaticus.
Word 2010 has a particular template that it uses when you first open the program. This template contains default styles, no text and you also use it when you create new, blank documents. As you customize styles, marcos and other elements these settings are automatically stored in this template. What is the name of this template file?
In error-prone PCR the Taq polymerase anneals incompatible base-pairs to each other during amplification.
Taq polymerase used in PCR is
Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease
DNA polymerase can distinguish between dNTPs and rNTPS due to ___________
How many active sites are present in the DNA polymerase to catalyze the addition of the four dNTPs?
The two divalent metal ions of the active sites of DNA polymerase are major catalytic elements that bring about changes required for the joining of the dNTPs.