Reverse Transcriptase PCR MCQ

Test your knowledge with important Reverse Transcriptase PCR MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Reverse Transcriptase PCR MCQs at Bissoy

• ________________ is a modification of RT – PCR.
  ক

  Overlap extension PCR

  খ

  Inverse PCR

  গ

  Thermal cycle sequencing PCR

  ঘ

  RACE
• The cDNA library provides several advantages over RT – PCR.
  ক

  True

  খ

  False
• From which organism is the enzyme reverse transcriptase isolated?
  ক

  Bacteria

  খ

  Fungi

  গ

  Virus

  ঘ

  Prions
• How many primers are used in the process of reverse transcriptase amplification?
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4
• The digestion of mRNA during RT – PCR is carried out by the enzyme ____________
  ক

  Exonuclease

  খ

  RNase H

  গ

  Bal 31

  ঘ

  Endonuclease
• In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________
  ক

  5’ end of RNA

  খ

  3’ end of RNA

  গ

  3’ end of cDNA

  ঘ

  5’ end of cDNA
• Which of the following serves as the first primer in RT – PCR for eukaryotic RNA?
  ক

  Oligo A

  খ

  Oligo T

  গ

  Oligo G

  ঘ

  Oligo C
• Reverse transcriptase produces DNA from RNA.
  ক

  True

  খ

  False