Bissoy.com
Doctors Medicines MCQs Q&A

In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________

In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________ Correct Answer 3’ end of cDNA

During the synthesis of the complementary strand for the cDNA molecule, its 3’ end is unknown. Thus no primer can be formed which can synthesize its complementary strand. Here the enzyme deoxynucleotydil transferase adds poly – G residues in the 3’ end of cDNA so that oligo C primer can be used for the synthesis of its complementary strand.

Related Questions

Consider the following statements for Poly-Si deposition: 1. Poly-Si layer is used for gate electrode of MOSFET because it has similar lattice constants with SiO2. 2. Poly-Si layer used for gate electrode of MOSFET for the better mechanical stability due to different thermal expansion coefficients. 3. In VLSI circuits, interconnects can be completed in one or two metal levels. 4. Poly-Si is used for short interconnects. Which of the above statements are correct ?
The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.
Chloroplast localization signals are also located in the __________ terminus and are about 25 to 100 residues in length, containing very few _______ charged residues but many hydroxylated residues such as serine.
Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________
A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme 'terminal transferase' The stock solution of dTTP that is used as a substrate has concentration of 150 μM. Ten μL of this stock solution is added to a total volume of 200 μL reaction. What will be the concentration of dTTP in the reaction?
If enzyme code EC 2.6.1.1 belongs to aspartate amino transferase, then the code EC 4.3.1.3 belongs to which enzyme?
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
What is the number of wattmeter required to measure the power of a poly phase of a poly phase system containing ‘n’ conductors?
How can the formulation of polynomial be done from its roots? ) c) poly() d) poly()
The effect of poly phase currents in poly phase winding can be compared to _______________