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Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________ Correct Answer colony PCR

When cells from colonies are taken and directly PCR is performed, it is called as colony PCR. Primers anneal to each side of the cloning site and there is no need of purification in such cases.

Related Questions

"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Which of the following statement(s) is/are CORRECT for antigen activated effector T cells?
P. CD4+ cells make contact with macrophages and stimulate their microbicidal activity.
Q. CD4+ cells make contact with B cells and stimulate them to differentiate into plasma cells.
R. CD8+ cells make contact with B cells and stimulate them to differentiate into plasma cells.
S. CD8+ cells make contact with virus infected cells and kill them.
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Dyslexia is a perceptual disorder often occurring in persons of normal, or even above average intelligence. The reader is unable to perceive correctly what is on a page. Letters and numbers often appear reversed: "b" seems to be "d","quite" is "quiet" and "from" is "form". The reader tends to leave out letters or words or insert words or letters that are not there. Vowel and consonant sounds may be confused. Many dyslexics are left­handed or able to write with either hand. They often confuse left and right. Learning to speak may also be delayed beyond infancy. The condition seems to be inherited. It may persist into adulthood. However, with early recognition and specialized approaches to teaching reading, most dyslexics can learn to read. Some researchers believe that latent dyslexia may be aggravated by the way reading is taught. The modern whole­word, or look­and­say, method seems to be more of a hindrance to learning for dyslexics than it is for ordinary pupils. The phonetic method of teaching students to learn letters and sound them out appears to achieve better reading results. The problem of words that cannot be sounded out ­ such as rough, laugh or through ­ is not solved by phonetics. These words must simply be memorized. However, for children with dyslexia the problem can be compounded by the failure of parents or teachers to recognize the condition. This can easily lead to emotional problems for dyslexic children, who cannot understand their failure to keep up with their classmates.
In Dyslexia, letters and figures often appear __________
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Dyslexia is a perceptual disorder often occurring in persons of normal, or even above average intelligence. The reader is unable to perceive correctly what is on a page. Letters and numbers often appear reversed: "b" seems to be "d","quite" is "quiet" and "from" is "form". The reader tends to leave out letters or words or insert words or letters that are not there. Vowel and consonant sounds may be confused. Many dyslexics are left­handed or able to write with either hand. They often confuse left and right. Learning to speak may also be delayed beyond infancy. The condition seems to be inherited. It may persist into adulthood. However, with early recognition and specialized approaches to teaching reading, most dyslexics can learn to read. Some researchers believe that latent dyslexia may be aggravated by the way reading is taught. The modern whole­word, or look­and­say, method seems to be more of a hindrance to learning for dyslexics than it is for ordinary pupils. The phonetic method of teaching students to learn letters and sound them out appears to achieve better reading results. The problem of words that cannot be sounded out ­ such as rough, laugh or through ­ is not solved by phonetics. These words must simply be memorized. However, for children with dyslexia the problem can be compounded by the failure of parents or teachers to recognize the condition. This can easily lead to emotional problems for dyslexic children, who cannot understand their failure to keep up with their classmates.
Dyslexia, often occurring in persons of normal, or even above average intelligence, is a __________
Given below are quantities named A,B and C. Based on the given information, you have to determine the relation between the quantities. You should use the given data and your knowledge of mathematics to choose among the possible answer. Akash’s present age is six years less than Badshah’s age after nine years. The ratio of present age of Badshahand RAFTAAR is 5:9. Raftaar is 21 years younger than Denny and Denny is three years older than Honey. Square of the Honey’s present age is 1350 more than 15 times of Honey’s present age? Quantity A: What is the difference between AKASH’s present age and HONEY’s age after 16 years? Quantity B: After 6 years Mankirat’s age is twice that of Akash’s present age. Diljeet’s present age is 3.5 times of 25% of sum of present age of Mankirat and A. What is the present age of Diljeet? Quantity C: Twice of A’s present age is eight years more than that of Babbu’spresent age, then find the Babbu’s present age?
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Dyslexia is a perceptual disorder often occurring in persons of normal, or even above average intelligence. The reader is unable to perceive correctly what is on a page. Letters and numbers often appear reversed: "b" seems to be "d","quite" is "quiet" and "from" is "form". The reader tends to leave out letters or words or insert words or letters that are not there. Vowel and consonant sounds may be confused. Many dyslexics are left­handed or able to write with either hand. They often confuse left and right. Learning to speak may also be delayed beyond infancy. The condition seems to be inherited. It may persist into adulthood. However, with early recognition and specialized approaches to teaching reading, most dyslexics can learn to read. Some researchers believe that latent dyslexia may be aggravated by the way reading is taught. The modern whole­word, or look­and­say, method seems to be more of a hindrance to learning for dyslexics than it is for ordinary pupils. The phonetic method of teaching students to learn letters and sound them out appears to achieve better reading results. The problem of words that cannot be sounded out ­ such as rough, laugh or through ­ is not solved by phonetics. These words must simply be memorized. However, for children with dyslexia the problem can be compounded by the failure of parents or teachers to recognize the condition. This can easily lead to emotional problems for dyslexic children, who cannot understand their failure to keep up with their classmates.
People suffering from dyslexia are often ___________
In the question two statements are given, followed by two conclusions, I and II. Read all the conclusions and then decide which of the given conclusions logically follow/s from the given statements. Statements: All white blood cells are red blood cells. Some blood cells are red blood cells. Conclusions: I. All blood cells are red blood cells, is a possibility. II. Some red blood cells are not blood cells.
The following sentences form a paragraph. The first and the last sentences of the paragraph are given. The rest of the sentences are numbered as P, Q, R and S. These four parts are not given in their proper order. Read the sentences and choose the alternative that arranges them in the correct order. 1. Sexual favouritism and gender partiality/discrimination are often similarly perceived. P. At the workplace, when romance involves sexual favouritism, it is important to comprehend the cause and extent of the presence of such favouritism i.e. whether this favouritism can give way to other employees (who are not directly involved in the illicit relationship) for claiming themselves as the victims of sexual harassment. Q. However, many critics have befittingly differentiated the two. R. This concludes that sexual favouritism and gender partiality bear both direct and indirect implications for employees.  S. They delineate that sexual favouritism entangles the discerning grant of a gain or opportunity at the workplace; in contrast, sexual partiality/discrimination usually involves the restraining of benefits or opportunities dependent upon an individual's gender. 6. Whether favouritism on the basis of sex remains a legitimate shape of discrimination, or whether sexual favouritism stretches to the extent of a restricted form of sexual partiality, is an important question which can only be answered through comprehending the definition of sexual harassment.
In a survey of 25 students, it was found that 15 had taken Mathematics, 12 had taken Physics and 11 had taken Chemistry, 5 had taken Mathematics and Chemistry, 9 had taken Mathematics and Physics, 4 had taken Physics and Chemistry and 3 had taken all the three subjects. Find the number of students who had taken both Physics and Chemistry but not Mathematics.
Read the passage carefully and choose the best answer to each question out of the four alternatives and click the button corresponding to it.
Dyslexia is a perceptual disorder often occurring in persons of normal, or even above average intelligence. The reader is unable to perceive correctly what is on a page. Letters and numbers often appear reversed: "b" seems to be "d","quite" is "quiet" and "from" is "form". The reader tends to leave out letters or words or insert words or letters that are not there. Vowel and consonant sounds may be confused. Many dyslexics are left­handed or able to write with either hand. They often confuse left and right. Learning to speak may also be delayed beyond infancy. The condition seems to be inherited. It may persist into adulthood. However, with early recognition and specialized approaches to teaching reading, most dyslexics can learn to read. Some researchers believe that latent dyslexia may be aggravated by the way reading is taught. The modern whole­word, or look­and­say, method seems to be more of a hindrance to learning for dyslexics than it is for ordinary pupils. The phonetic method of teaching students to learn letters and sound them out appears to achieve better reading results. The problem of words that cannot be sounded out ­ such as rough, laugh or through ­ is not solved by phonetics. These words must simply be memorized. However, for children with dyslexia the problem can be compounded by the failure of parents or teachers to recognize the condition. This can easily lead to emotional problems for dyslexic children, who cannot understand their failure to keep up with their classmates.
The problem of perception can be compounded by the failure of parents and teachers to