Genetic Engineering MCQ

Test your knowledge with important Genetic Engineering MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Genetic Engineering MCQs at Bissoy

• For a convenient transformation system, _____ can be used for gene silencing.
  ক

  antisense RNA

  খ

  transposon insertion

  গ

  either antisense RNA or transposon insertion

  ঘ

  transposon insertion followed by antisense RNA
• The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________
  ক

  reflects, can be determined

  খ

  reflects, can’t be determined

  গ

  doesn’t necessarily reflects, can be determined

  ঘ

  doesn’t necessarily reflects, can’t be determined
• If a mutation perturbs the structure, then stability and folding are not affected.
  ক

  True

  খ

  False
• For getting a large amount of proteins to crystallize, which of the following should be used as an expression system?
  ক

  Bacterial system

  খ

  Yeast systems

  গ

  Eukaryotic systems

  ঘ

  Both eukaryotic and bacterial systems can be used
• If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct?
  ক

  The protein is not protease

  খ

  The protein can be incorrectly folded which can block the protease activity

  গ

  There might be some other cofactor required for protease activity

  ঘ

  The most commonly used expression system is E.coli
• If a full proteomic analysis of growth medium is carried out and is combined with ____________ genome sequence, genes for other _____________ proteins are also obtained.
  ক

  partial, defensive

  খ

  partial, secreted

  গ

  complete, defensive

  ঘ

  complete, secreted
• How many approaches are there in order to clone the complete genome?
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4
• The disadvantage in the approach based on using PCR is that there is no permanent record until some of the bacterial cells are preserved.
  ক

  True

  খ

  False
• Which of the following is not suitable if the PCR product is non-specific?
  ক

  Adjusting the concentration of magnesium ions

  খ

  Increasing annealing temperature

  গ

  Using touchdown PCR

  ঘ

  Using inverse PCR
• Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________
  ক

  non-specific, directly

  খ

  non-specific, indirectly or directly

  গ

  specific, directly

  ঘ

  specific, indirectly
• For carrying out gene manipulation, the use of cultured cells is _____ transgenic organisms.
  ক

  less reliable

  খ

  more reliable

  গ

  may be less or more reliable

  ঘ

  is same reliable as
• If controlled inactivation of a gene is carried out and some of the consequences when inactivation of a target gene is deleterious are avoided. It is referred as ____________
  ক

  specialized gene targeting

  খ

  controlled gene targeting

  গ

  conditional gene targeting

  ঘ

  specific gene targeting
• The gene targeting approach produces individuals which are ______ for inactivation of the gene.
  ক

  homozygous

  খ

  heterozygous

  গ

  either only homozygous or only heterozygous

  ঘ

  both heterozygous and homozygous
• It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting.
  ক

  True

  খ

  False
• Integration events may be insertional involving _____ crossover or replacement involving _____ crossovers.
  ক

  single, single

  খ

  double, double

  গ

  double, single

  ঘ

  single, double
• If a gene is inactivated by gene targeting then it is called as ____________
  ক

  knock-in gene

  খ

  knock-out gene

  গ

  gene disruption

  ঘ

  insertional inactivation
• ES cells are used in order to ensure that insertion is done at the required chromosomal location and it is called as ____________
  ক

  gene targeting

  খ

  knocking out

  গ

  knocking in

  ঘ

  gene disruption
• Embryonic stem cells (ES) are isolated and are injected again into the blastocoel of a developing embryo. The embryo which develops is entirely made up of these cells only.
  ক

  True

  খ

  False
• Embryonic stem cells are also used for generation of transgenic organisms. They are obtained from _______ of a developing ________
  ক

  trophoectoderm, gastrula

  খ

  trophoectoderm, blastula

  গ

  inner cell mass, blastula

  ঘ

  inner cell mass, gastrula
• If the embryo is at one-cell stage then it is found in ____________
  ক

  ovary

  খ

  oviduct

  গ

  uterus

  ঘ

  either ovary or uterus
• The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.
  ক

  depends only on, not depends

  খ

  depends, depends also

  গ

  doesn’t depends, but depends

  ঘ

  neither depends, nor depends
• There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.
  ক

  cysteine

  খ

  methionine

  গ

  either cysteine or methionine

  ঘ

  cysteine and methionine both
• Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).
  ক

  inducible, short terminal repeat region

  খ

  inducible, long terminal repeat region

  গ

  constitutive, short terminal repeat region

  ঘ

  constitutive, long terminal repeat region
• Expression of tTA gene is ________ by doxycycline which is _______ analogue.
  ক

  activated, tetracycline

  খ

  inactivated, chloramphenicol

  গ

  activated, chloramphenicol

  ঘ

  inactivated, tetracycline
• Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).
  ক

  True

  খ

  False
• In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.
  ক

  presence, activates

  খ

  absence, activates

  গ

  absence, inactivates

  ঘ

  presence, inactivates
• Constitutive promoters are majorly obtained from ________
  ক

  fungi

  খ

  bacteria

  গ

  mammalian cells

  ঘ

  viral cells
• Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.
  ক

  alpha satellite

  খ

  beta satellite

  গ

  satellite 2

  ঘ

  satellite 1
• Choose the incorrect statement in respect to MACs.
  ক

  They are mammalian or human artificial chromosomes

  খ

  Large stretches of DNA (in excess of 100 k[ can be inserted

  গ

  A selectable marker is required

  ঘ

  It can be maintained for long term
• The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?
  ক

  Deletion of the packaging signal in the helper genome

  খ

  Deletion of multiple parts of the helper genome

  গ

  Division of the helper genome into several pieces

  ঘ

  All of the mentioned