It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting.

Which of the following statement(s) is/are CORRECT for antigen activated effector T cells?
P. CD4+ cells make contact with macrophages and stimulate their microbicidal activity.
Q. CD4+ cells make contact with B cells and stimulate them to differentiate into plasma cells.
R. CD8+ cells make contact with B cells and stimulate them to differentiate into plasma cells.
S. CD8+ cells make contact with virus infected cells and kill them.

A

Q only

B

Q and S only

C

P, Q and S only

D

P, Q, R and S

The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps: i) Peeling of membranes carrying away bacterial cells with it ii) Cells are lysed and denaturing of DNA is being carried out iii) Hybridization with labelled DNA iv) Placing the membrane onto plate containing recombinant cells Choose the correct sequence in which the steps are carried out (starting to ending).

A

iv)-i)-ii)-iii)

B

iv)-ii)-i)-iii)

C

i)-ii)-iii)-iv)

D

iii)-ii)-i)-iv)

Northern Blotting is not a variant of Southern blotting.

A

True

B

False

Which of the following is found in Northern blotting but not in southern blotting?

A

Gel

B

Nitrocellulose

C

Reactive paper

D

Probe

Consider the following statements.
1. The first transgenic cow is Rosie.
2. The milk of first transgenic cow contained the human alpha-lactalbumin.
Which of the statements given above is/are correct about transgenic cow?

A

Both 1 and 2

B

Only 1

C

Only 2

D

Neither 1 nor 2

Which set of the following events occurs during the elongation step of translation?
P. Attachment of mRNA with the smaller subunit of ribosome
Q. Loading of correct aminoacyl-tRNA into the A site.
R. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P site.
S. Dissociation of the ribosomal subunits.
T. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome.

A

P, Q and R

B

P, Q and T

C

Q, R and T

D

R, S and T

Which of the following statements are correct regarding common differences between plant and animal cells?
1. Plant cells have cellulose cell wall, while animal cells lack it.
2. Plant cells lack plasma membrane, while animal cells have it.
3. Mature plant cell have a large vacuole, while animal cells have numerous small vacuoles.

A

1 and 2

B

2 and 3

C

1 and 3

D

1, 2 and 3

The genes carried by transgenic animals make them more ______ to toxic substances as compared to non-transgenic animals.

A

strong

B

sensitive

C

infective

D

resistant

You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because

A

There are no introns in the genome

B

The introns contain the recognition sites and have already been spliced out

C

All of restriction fragments are too small to detect

D

Restriction endonucleases do not cut RNA, and this virus has an RNA genome

The inlet to the heatbox must confirm an odd fiber slurry consistency and pressure across the width of the heatbox to confirm cross direction non-uniformity of paper.

A

True

B

False

It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting.

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