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Five friends went for a trip where each carried a certain number of apples with them. The number of apples carried by C was 1 less than that by D, which was 5 more than the number of apples carried by E. The total number of apples carried by A and B both was equal to the average number of apples carried by C, D and E. If D carried more apples than B and they together carried 14 apples, which of the following is a possible value of the apples carried by A?
If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?
Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________
cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).
You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________
A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?
The enzyme that degrades the mRNA recognizes ____________ bond of mRNA.
Analysis of a hexapeptide using enzymatic cleavage reveals the following result:
• Amino acid composition of the peptide is: 2R, A, V, S, Y
• Trypsin digestion yields two fragments and the compositions are: (R, A, V) and (R, S, Y)
• Chymotrypsin digestion yields two fragments and the compositions are: (A, R, V, Y) and (R, S)
• Digestion with carboxypeptidase A yields no cleavage product.
Given: Trypsin cleaves at carboxyl side of R. Chymotrypsin cleaves at carboxyl side of Y. Carboxypeptidase A cleaves at amino side of the C-terminal amino acid (except R and K) of the peptide.
The correct amino acid sequence of the peptide is:
"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________