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You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________ Correct Answer 2nd strand

mRNA produced from 1st strand and DNA produced by replicating 1st strand should have the same sequence and they will not hybridize. However, if the gene is transcribed from 2nd strand then this result is possible. RNA pol only transcribes one strand at a time so dispersed and both being transcribed are not possible.

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