Which of the following serves as the first primer in RT – PCR for eukaryotic RNA?
Which of the following serves as the first primer in RT – PCR for eukaryotic RNA? Correct Answer Oligo T
All RNAs have a poly – A tail added to its 3’ end as the post transcriptional modification. Thus, during reverse transcription this poly – A tail is used as the template strand and a primer of oligo T that is used for the synthesis of the cDNA strand.
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Feb 20, 2025