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PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer. Correct Answer True

Megaprimer is produced when amplification is carried out by using mutagenic primer and one of the flanking primers. It corresponds to the part of the gene.

Related Questions

Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________
If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification?
If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification?
Both the primers, the start primer and the end primer should have a nearly same melting temperature.
Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'
Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor
The DNA sequence shown below is to be amplified by PCR:
5' GCTAAGATCTGAATTTTCC . . . . . . . .
TTGGGCAATAATGTAGCGC 3'
3' CGATTCTAGACTTAAAAGG . . . . . . . .
AACCCGTTATTACATCGCG 5'
Which one of the following pair of primers can be used for this amplification?
A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.