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Both the primers, the start primer and the end primer should have a nearly same melting temperature.

Both the primers, the start primer and the end primer should have a nearly same melting temperature. Correct Answer True

Both the primers should have a nearly same melting temperature. It is so because then they would nearly bind at the same time as the temperature is being lowered for their annealing.

Related Questions

Which row of the following table correctly matches the terms with the respective processes of state-change?   Change of State   Gas to liquid Liquid to solid Solid to liquid 1. Condensation Melting Solidification 2. Condensation Solidification Melting 3. Evaporation Melting Solidification 4. Evaporation Solidification Melting
Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________
Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'
The Indian rupee for the first time weakened past the 73 mark against the US dollar after the crude oil prices surged to nearly $85 per barrel. INR is currently the worst performing Asian currency as India imports nearly 80% of its fuel needs. Which of the following is not-in-line with the above statement? I: The crude oil prices surged to nearly $85 per barrel. II: The Indian rupee for the first time weakened past the 73 mark against the US dollar. III: India exports nearly 80% of its fuel needs.
Two long rods A and B of the same diameter have thermal conductivities k and 4k and have one of their end inserted into a furnace at 400 K. At 9.5 m away section from the furnace, the temperature of rod B is120 degree Celsius. So find out at what end from the furnace end, the same temperature would be reached in the rod A?
PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.
The given conditions are imposed by an officer for providing the Travelling Concession to the employees. The employee A. Should have made 2 trips in a 4 calendar years. B. Should have made one trip in 1 calendar year. C. Should have made one trip within the native country, while the other could have been overseas. D. Should have made a trip with maximum of 6 people. E. The total cost of both the trips should not have exceeded Rs. 6 lakhs. In case of employee who satisfies all the other criteria except: I. At (D) above, he/she would get Rs. 4 lakhs as concession. II. At (E) above, he/she would get Rs. 3 lakhs as concession. In the inquiry underneath, data of one employee is given. You need to take one of the accompanying four choices in the light of the data given and the criteria and conditions given above. Shyam, a native of India, along with his wife and 2 children went for vacations within India. He made his first trip in February, 2016 and second one in August 2016. The total expenditure of the trips were four lakhs and forty thousand.
Two refrigerators M and N operate in series. The refrigerator M absorbs energy at the rate of 1 kW from a body at temperature 400 K and rejects energy as heat to a body at a temperature T. The refrigerator N absorbs the same quantity of energy which is rejected by the refrigerator N from the body at temperature T, and rejects energy as heat to a body at temperature 800 K. If both the refrigerators have the same C.O.P., what is the value temperature T?
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Consider a Simple Checkpointing Protocol and the following set of operations in the log. (start, T4); (write, T4, y, 2, 3); (start, T1); (commit, T4); (write, T1, z, 5, 7); (Checkpoint); (start, T2); (write, T2, x, 1, 9); (commit, T2); start, T3); (write, T3, z, 7, 2); if a crash happens now and the system tries to recover using both undo and redo operations, what are the contents of the undo list and the redo list?