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Thermostable DNA polymerases are very important in PCR. How are they obtained?

Thermostable DNA polymerases are very important in PCR. How are they obtained? Correct Answer They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents

Thermostable DNA polymerases are found naturally in thermophilic bacteria which can be found growing in oceanic vents. They are having high stability and their DNA polymerases can function effectively even at high temperatures in-vitro.

Related Questions

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
You wanted to find out the importance of the DNA polymerases. So, you mutated the polymerases one by one and checked for survival. In which of these mutant cases will you see least survival rate?
Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________
If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification?
If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification?
Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?
Bacterial polymerases are slower than eukaryotic polymerases.
All the RNA polymerases share 5 common subunits, which are present in all the three polymerases and are all alike. State whether the statement is true or false.
The ratio of marks obtained by Rohit in Mathematics to the marks obtained in Science is 8 : 7, the marks of English is 10% more than the marks obtained in Science and the marks obtained in Hindi is 10% less than the marks obtained in Mathematics. If each subject is of 100 marks and the sum of marks of English and Hindi is 149, then find the percentage of passing mark where the passing marks is 40 more than the marks obtained by Rohit in Mathematics.