Polymerases MCQ

Test your knowledge with important Polymerases MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Polymerases MCQs at Bissoy

• Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?
  ক

  They only add a single nucleotide

  খ

  They only add a string of nucleotides and not a single nucleotide

  গ

  Terminal transferase adds a series of nucleotides at the 3’ end

  ঘ

  Taq polymerase adds a single nucleotide at the 5’ end of the PCR product
• Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?
  ক

  RNA dependent DNA polymerase

  খ

  DNA dependent RNA polymerase

  গ

  RNA dependent RNA polymerase

  ঘ

  DNA dependent DNA polymerase
• Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?
  ক

  Because they are not having 3’-5’ proofreading exonucleolytic activity

  খ

  Because they are not having 5’-3’ proofreading exonucleolytic activity

  গ

  Because they are having slow rates of exonucleolytic activity

  ঘ

  It is difficult to synthesize DNA from RNA
• Which of the following enzyme is said as reverse transcriptase?
  ক

  DNA dependent DNA polymerase

  খ

  RNA dependent RNA polymerase

  গ

  RNA dependent DNA polymerase

  ঘ

  DNA dependent RNA polymerase
• Thermostable DNA polymerases are very important in PCR. How are they obtained?
  ক

  They are obtained by heating the bacteria manually over high temperatures

  খ

  They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents

  গ

  They are found everywhere in nature

  ঘ

  They are obtained by genetically modifying the E. coli bacteria with thermal stability property
• Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________
  ক

  polishing and end filling respectively

  খ

  end filling and polishing respectively

  গ

  polishing in both the cases

  ঘ

  end filling in both the cases
• The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated?
  ক

  The smaller fragment is having C terminal and the larger fragment is having N terminal

  খ

  The smaller fragment is named as Klenow fragment and the intact molecule is called as Kornberg fragment

  গ

  The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity

  ঘ

  Both the fragments are having 5’-3’ polymerase and 3’-5’ exonuclease activity
• Which of the following activity is not possible in the case of DNA polymerase I?
  ক

  3’-5’ exonuclease

  খ

  5’-3’ exonuclease

  গ

  5’-3’ DNA synthesis

  ঘ

  3’-5’ DNA synthesis
• Polymerase can be defined as ______________
  ক

  an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand

  খ

  an enzyme used for removal of nucleotides from the DNA or RNA strand

  গ

  an enzyme which can synthesize only a new DNA strand, not an RNA strand

  ঘ

  an enzyme which can synthesize either a new DNA or an RNA strand but only when a strand is there
• Basic classification of polymerases includes how many types?
  ক

  2

  খ

  3

  গ

  4

  ঘ

  5