Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?
Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases? Correct Answer Because they are not having 3’-5’ proofreading exonucleolytic activity
Reverse transcriptase enzymes are not having proof reading exonuclease activity in 3’-5’ direction. Hence, when the synthesis is done in 5’-3’ direction it is not checked in the 3’-5’ direction and thus the errors inculcated won’t be removed.
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Feb 20, 2025