1 Answers
In enzymology, phosphoribosylamine—glycine ligase, also known as glycinamide ribonucleotide synthetase , is an enzyme that catalyzes the chemical reaction
which is the second step in purine biosynthesis.The 3 substrates of this enzyme are ATP, 5-phospho-D-ribosylamine, and glycine, whereas its 3 products are ADP, phosphate, and N1-glycinamide.
This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds.
In bacteria, GARS is a monofunctional enzyme. The purD genes often contain PurD RNA motif in their 5' UTR. In yeast, GARS is part of a bifunctional enzyme in conjunction with phosphoribosylformylglycinamidine cyclo-ligase. In higher eukaryotes, including humans, GARS is part of a trifunctional enzyme in conjunction with AIRS and with phosphoribosylglycinamide formyltransferase , forming GARS-AIRS-GART.