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In enzymology, a cysteine—tRNA ligase is an enzyme that catalyzes the chemical reaction

The 3 substrates of this enzyme are ATP, L-cysteine, and tRNA, whereas its 3 products are AMP, diphosphate, and L-cysteinyl-tRNA.

This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-cysteine:tRNACys ligase. Other names in common use include cysteinyl-tRNA synthetase, cysteinyl-transferRNA synthetase, cysteinyl-transfer ribonucleate synthetase, and cysteine translase. This enzyme participates in cysteine metabolism and aminoacyl-trna biosynthesis.

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