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In enzymology, a lysine—tRNA ligase is an enzyme that catalyzes the chemical reaction

The 3 substrates of this enzyme are ATP, L-lysine, and tRNA, whereas its 3 products are AMP, diphosphate, and L-lysyl-tRNA.

This enzyme participates in 3 metabolic pathways: lysine biosynthesis, aminoacyl-trna biosynthesis, and amyotrophic lateral sclerosis.

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