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In enzymology, a D-alanine—poly ligase is an enzyme that catalyzes the chemical reaction
The 3 substrates of this enzyme are ATP, D-alanine, and poly, whereas its 3 products are AMP, diphosphate, and O-D-alanyl-poly.
This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is D-alanine:poly ligase. Other names in common use include D-alanyl-poly synthetase, D-alanine: membrane acceptor ligase, D-alanine-D-alanyl carrier protein ligase, D-alanine-membrane acceptor ligase, and D-alanine-activating enzyme. This enzyme participates in d-alanine metabolism.