Which of the following is incorrect about Purification of protein complexes followed by mass spectrometry?

Which of the following is incorrect about Purification of protein complexes followed by mass spectrometry? Correct Answer UnLike in the yeast-two-hybrid assay, this does not involve chimeric genes

Like in the yeast-two-hybrid assay, this is done by making chimeric genes that are introduced into cells. The principle of mass spectrometric identification of proteins is that the protein is chopped into fragments by tryptic digestion, and the mass of each fragment is measured by matrix-assisted laser desorption/ ionization-time-of-flight mass spectrometry (MALDI-TOF MS). This measurement is so accurate that the combination of amino acids in each fragment can be calculated and compared to a database of all the proteins in the proteome of the organism in order to find the correct one.
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Related Questions

An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.
What is the monoisotopic and average mass of valine? Given data: Mass of the most abundant isotope of Carbon (C°)=12.0000, Mass of most abundant isotope of Hydrogen (H°)=1.0078, Mass of most abundant isotope of Oxygen (O°)=15.9949, Mass of most abundant isotope of Nitrogen (N°)=14.0031, The average mass of Carbon (C)=12.011, The average mass of Hydrogen (H)=1.008, The average mass of Oxygen (O)=15.999, The average mass of Nitrogen (N)=14.007.
Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
Directions : In each of the following questions a statement is given, followed by two conclusions. Give answer :

Statements : About 50 per cent of the animal by-products - hair, skin, horns etc. is edible protein. American chemists have developed a method of isolating 45 per cent of this protein. They used an enzyme developed in Japan to break down soya protein.

Conclusions :
I. Americans have not been able to develop enzymes.
II. Animal by-products protein has the same composition as soya protein.
In Proteolysis and fragment sequencing, Protein spots may be excised from a two-dimensional protein gel and subjected to a combination of amino acid sequencing and cleavage analyses using the techniques of mass spectrometry and high-pressure liquid chromatography.
Protein-protein interactions are studied by
P. DNA foot printing
Q. Yeast two hybrid system
R. Ligase chain reaction
S. Mass spectrometry
Which of the following is incorrect regarding Mass Spectrometry Protein Identification?
Identify the correct relation between Δo and Δt, where Δo denotes crystal field splitting in octahedral complexes and Δt denotes crystal field splitting in tetrahedral complexes.
The ligand field bands of lanthanide complexes are generally sharper than those of transition metal complexes because
Protein A will fold into its native state only when protein B is also present in the solution. However, protein B can fold itself into native confirmation without the presence of protein A. Which of the following is true?