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An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column. Correct Answer True

The purified proteins are then analyzed by gel electrophoresis followed by MS for identification of the interacting components. The protein microarray systems mentioned above also provide a high throughput alternative for studying protein–protein interactions.

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