Protein Interactions MCQ

Test your knowledge with important Protein Interactions MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Protein Interactions MCQs at Bissoy

• The most detailed experimental information about protein-protein interactions comes from three-dimensional structures.
  ক

  True

  খ

  False
• Structural analyses on small sets of proteins have shown that the domains from a pair of families bind to each other with the same geometry in multi-domain proteins and in transient interactions.
  ক

  True

  খ

  False
• In the phylogenetic profile method for predicting protein interaction, presence or absence of orthologous genes is scored across a variety of genomes.
  ক

  True

  খ

  False
• In the assessment of methods to predict protein-protein interactions, one third of such pairs were found to physically interact, and an additional third to belong to the same metabolic pathway or functional process.
  ক

  True

  খ

  False
• The phylogenetic profile method relies on detection of orthologs (or homologs, in a variation of the method) in a set of genomes.
  ক

  True

  খ

  False

  গ

  11. The phylogenetic profile method relies on detection of orthologs (or homologs, in a variation of the metho

  ঘ

  in a set of genomes. a) True b) False
• Due to the requirement for co-regulation as well as colocalization, the method is mostly limited to certain classes of protein-protein interactions.
  ক

  True

  খ

  False
• Domains that are part of a multidomain protein are ______
  ক

  nethier co-regulated nor colocalized

  খ

  not co-regulated but colocalized

  গ

  co-regulated and but not colocalized

  ঘ

  co-regulated and colocalized
• An approach for predicting ______ to look for cases across a set of genomes where _____ are part of the same gene in one genome resulted in gene fusion method.
  ক

  gene interactions, only three to four orthologs

  খ

  gene interactions, two orthologs

  গ

  protein interactions, two or more orthologs

  ঘ

  protein interactions, two orthologs
• Conservation of gene order due to operon structure is _______ so interactions of proteins specific to eukaryotes cannot be detected by method of Conservation of gene order.
  ক

  not applicable to archea genomes

  খ

  not applicable to prokaryote genomes

  গ

  applicable to eukaryote genomes

  ঘ

  not applicable to eukaryote genomes
• In a quantitative assessment of this method (Conservation of gene order) using the genome of the parasitic organism Mycoplasma genitalium as a benchmark.
  ক

  True

  খ

  False
• Members of a stable complex are often co-regulated and thus will be detected by the method of Conservation of gene order.
  ক

  True

  খ

  False
• When comparing pairs of genes or sets of genes in different genomes for this purpose, it is not mandatory for the genes to be orthologs.
  ক

  True

  খ

  False
• Genes that are consistently part of the same operon across different, distantly related genomes are likely to be part of the same protein complex or functional process across all species.
  ক

  True

  খ

  False
• Interactions between proteins can be predicted computationally by looking for sets of genes that occur as a _______
  ক

  single gene in at least one genome

  খ

  multiple genes in at least one genome

  গ

  multiple genes in various genomes

  ঘ

  single gene in various genomes
• Experimentation is most desirable over computational methods by every means.
  ক

  True

  খ

  False
• Which of the following is incorrect about Purification of protein complexes followed by mass spectrometry?
  ক

  Isolating protein complexes from cells allows identification of interactions between ensembles of proteins instead of just pairs

  খ

  Systematic purification of complexes on a large scale is done by tagging hundreds of genes with an epitope

  গ

  UnLike in the yeast-two-hybrid assay, this does not involve chimeric genes

  ঘ

  Affinity purification based on the epitope will then extract all the proteins attached to the bait protein from cell lysates
• Which of the following is incorrect about Yeast-two-hybrid screens?
  ক

  The yeast-two-hybrid system uses the transcription of a reporter gene driven by the Gal4 transcription factor to monitor whether or not two proteins are interacting

  খ

  The DNA-binding domain chimeric protein will not bind upstream of the reporter gene

  গ

  If the activation domain chimeric protein interacts with the DNA-binding domain chimeric protein, the reporter gene will be transcribed

  ঘ

  Disadvantages of the method are that only pairwise interactions are tested, and not interactions that can only take place when multiple proteins come together, as well as a high false positive rate
• For proteins in stable complexes the average sequence identity is 46%, while for proteins in transient interactions it is 41%.
  ক

  True

  খ

  False
• Membership in a stable complex also differs from transient interaction in terms of evolutionary constraints upon sequence divergence.
  ক

  True

  খ

  False
• Correlation of gene expression for pairs of transiently interacting proteins is ______ compared to randomly chosen pairs of proteins.
  ক

  not significant

  খ

  only marginally significant

  গ

  totally significant

  ঘ

  significant to much extent
• Sets of proteins that are part of stable complexes and sets of proteins involved in transient interactions ____ in terms of the similarity in gene expression among the set of proteins.
  ক

  are similar

  খ

  differ

  গ

  are same

  ঘ

  show similar function
• Stable complexes consist of proteins that are _____ associated with each other, like many ____ proteins for instance.
  ক

  temporarily, oligomeric

  খ

  temporarily, monomeric

  গ

  permanently, oligomeric

  ঘ

  permanently, monomeric
• There exist three types of interactions between domains. Which of the following is not one of them?
  ক

  Stable complex

  খ

  Transient interaction

  গ

  Multi-domain protein

  ঘ

  Unstable interaction
• The physical contacts between domains are crucial for the functioning of the cellular machinery.
  ক

  True

  খ

  False
• Which of the following is untrue regarding the STRING?
  ক

  Search Tool for the Retrieval of Interacting Genes/Proteins

  খ

  Functional associations include only the direct protein-protein interactions

  গ

  It is based on combined evidence of gene linkage, gene fusion and phylogenetic profiles

  ঘ

  It is a web server that predicts gene and protein functional associations
• Which of the following is untrue regarding the predicting Interactions Based on Phylogenetic Information?
  ক

  Proteins do not operate as a complex

  খ

  This method detects the co-presence or co-absence of orthologs across a number of genomes

  গ

  Protein interactions can be predicted using phylogenetic profiles

  ঘ

  Phylogenetic profile are defined as patterns of gene pairs that are concurrently present or absent across genomes
• In Predicting Interactions Based on Gene Neighbors if a certain gene linkage is found to be indeed conserved across divergent genomes, it can be used as a strong indicator of formation of an operon that encodes proteins that are functionally and even physically coupled.
  ক

  True

  খ

  False
• The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function.
  ক

  distant

  খ

  close

  গ

  extremely distant

  ঘ

  extremely close
• When the two domains are located in two different proteins, to preserve the same functionality, their close proximity and interaction have to be preserved as well.
  ক

  True

  খ

  False
• Which of the following is untrue regarding the Predicting Interactions Based on Domain Fusion?
  ক

  It is based on gene fusion events

  খ

  Predicting protein–protein interactions is called the “Rosetta stone” method

  গ

  A fused protein often reveals relationships between its domain components

  ঘ

  A fused protein doesn’t necessarily reveal about the relationships between its domain components