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Which of the following is incorrect about Yeast-two-hybrid screens?

Which of the following is incorrect about Yeast-two-hybrid screens? Correct Answer The DNA-binding domain chimeric protein will not bind upstream of the reporter gene

If the interaction between two proteins, A and B, is being tested, one of their genes would be fused to the DNA-binding domain of the Gal4 transcription factor (Gal4-DBD) while the other would be fused to the activation domain (Gal4-AD). The DNA-binding domain chimeric protein will bind upstream of the reporter gene. This experiment can be carried out hundreds or even thousands of times on microassay plates, as in the case of the study by Uetz and colleagues on S.cerevisiae (yeast) interactions. Each array element on these plates contains yeast cells transformed with a particular combination of two plasmids, one carrying the DNA-binding domain chimeric protein and the other the activation domain chimeric protein.

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