Protein MCQ

Test your knowledge with important Protein MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Protein MCQs at Bissoy

• Which of the following is incorrect about Purification of protein complexes followed by mass spectrometry?
  ক

  Isolating protein complexes from cells allows identification of interactions between ensembles of proteins instead of just pairs

  খ

  Systematic purification of complexes on a large scale is done by tagging hundreds of genes with an epitope

  গ

  UnLike in the yeast-two-hybrid assay, this does not involve chimeric genes

  ঘ

  Affinity purification based on the epitope will then extract all the proteins attached to the bait protein from cell lysates
• Which of the following is incorrect about Yeast-two-hybrid screens?
  ক

  The yeast-two-hybrid system uses the transcription of a reporter gene driven by the Gal4 transcription factor to monitor whether or not two proteins are interacting

  খ

  The DNA-binding domain chimeric protein will not bind upstream of the reporter gene

  গ

  If the activation domain chimeric protein interacts with the DNA-binding domain chimeric protein, the reporter gene will be transcribed

  ঘ

  Disadvantages of the method are that only pairwise interactions are tested, and not interactions that can only take place when multiple proteins come together, as well as a high false positive rate
• For proteins in stable complexes the average sequence identity is 46%, while for proteins in transient interactions it is 41%.
  ক

  True

  খ

  False
• Membership in a stable complex also differs from transient interaction in terms of evolutionary constraints upon sequence divergence.
  ক

  True

  খ

  False
• Correlation of gene expression for pairs of transiently interacting proteins is ______ compared to randomly chosen pairs of proteins.
  ক

  not significant

  খ

  only marginally significant

  গ

  totally significant

  ঘ

  significant to much extent
• Sets of proteins that are part of stable complexes and sets of proteins involved in transient interactions ____ in terms of the similarity in gene expression among the set of proteins.
  ক

  are similar

  খ

  differ

  গ

  are same

  ঘ

  show similar function
• Stable complexes consist of proteins that are _____ associated with each other, like many ____ proteins for instance.
  ক

  temporarily, oligomeric

  খ

  temporarily, monomeric

  গ

  permanently, oligomeric

  ঘ

  permanently, monomeric
• There exist three types of interactions between domains. Which of the following is not one of them?
  ক

  Stable complex

  খ

  Transient interaction

  গ

  Multi-domain protein

  ঘ

  Unstable interaction
• The physical contacts between domains are crucial for the functioning of the cellular machinery.
  ক

  True

  খ

  False