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It has been estimated that up to 11% of cDNA clones may be chimeric.

It has been estimated that up to 11% of cDNA clones may be chimeric. Correct Answer True

A problem of ESTs is the presence of chimeric clones owing to cloning artifacts in library construction, in which more than one transcript is ligated in a clone resulting in the 5_ end of a sequence representing one gene and the 3’ end another gene. Another fundamental problem with EST profiling is that it predominantly represents highly expressed, abundant transcripts. Weakly expressed genes are hardly found in a EST sequencing survey.

Related Questions

cDNA made from the mRNA of an organism was used to make a cDNA library in a vector that allows the expression as a fusion with a reporter tag. What percentage of the cDNA clones is likely to give rise to correct gene products?
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cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).
To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts.
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