Based Approaches MCQ

Test your knowledge with important Based Approaches MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30 + more Based Approaches MCQs at Bissoy

• Which of the following is untrue regarding SAGE?
  ক

  It stands for Serial analysis of gene expression

  খ

  It is another high throughput, sequence-based approach for global gene expression profile analysis

  গ

  It stands for Squared analysis of gene expression

  ঘ

  Unlike EST sampling, SAGE is more quantitative in determining mRNA expression in a cell
• Which of the following is untrue regarding TIGR Gene Indices?
  ক

  It is an EST database that the similar type of clustering method from UniGene

  খ

  It is an EST database that uses a different clustering method from UniGene

  গ

  It compiles data from dbEST, GenBank mRNA and genomic DNA data, and TIGR’s own sequence databased Sequences are only clustered if they are more than 95% identical for over a fortynucleotide region in pairwise comparisons

  ঘ

  None of the mentioned
• Which of the following is untrue regarding UniGene?
  ক

  It is an NCBI EST cluster database

  খ

  Overlapping EST sequences are computationally processed to represent a single expressed gene

  গ

  Each cluster is a set of overlapping EST sequences

  ঘ

  The overlapping EST sequences are computationally processed to represent a set of expressed genes
• Which of the following is untrue regarding EST Index Construction?
  ক

  The goal of the EST databases is to organize and consolidate the largely redundant EST data

  খ

  The process includes a preprocessing step that removes masks repeats

  গ

  There is no screening of vector contaminants

  ঘ

  The goal of the EST databases is to improve the quality of the sequence information so the data can be used to extract full-length cDNAs
• GenBank has a special EST database, dbEST that contains EST collections for a large number of organisms.
  ক

  True

  খ

  False
• It has been estimated that up to 11% of cDNA clones may be chimeric.
  ক

  True

  খ

  False
• Which of the following is untrue regarding the drawbacks of expressed sequence tags (ESTs)?
  ক

  They are often of lowquality because they are automatically generated without verification

  খ

  Many bases are ambiguously determined, represented by N’s

  গ

  Frame shift errors and artifactual stop codons are some common errors

  ঘ

  Despite of all the failures, the translation the sequences is smooth
• To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts.
  ক

  True

  খ

  False
• Which of the following is untrue regarding expressed sequence tags (ESTs)?
  ক

  One of the high throughput approaches to genome-wide profiling of gene expression is sequencing ESTs

  খ

  They are short sequences obtained from cDNA clones

  গ

  They serve as short identifiers of full-length genes

  ঘ

  They are typically in the range of 800 to 900 nucleotides in length