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To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts.

To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts. Correct Answer True

The EST data are able to provide a rough estimate of genes that are actively expressed in a genome under a particular physiological condition. This is because the frequencies for particular ESTs reflect the abundance of the corresponding mRNA in a cell, which corresponds to the levels of gene expression at that condition. Another potential benefit of EST sampling is that, by randomly sequencing cDNA clones, it is possible to discover new genes.

Related Questions

cDNA made from the mRNA of an organism was used to make a cDNA library in a vector that allows the expression as a fusion with a reporter tag. What percentage of the cDNA clones is likely to give rise to correct gene products?
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