Which of the following is not supposed to be present in the PCR reaction mixture solution?

Which of the following is not supposed to be present in the PCR reaction mixture solution? Correct Answer Calcium ions

Calcium ions are not supposed to be present in the PCR reaction mixture solution. Deoxynucleotide triphosphates (dNTPs), magnesium ions, and buffers are supposed to be present in the PCR reaction mixture solution. dNTPs are the building blocks from which the DNA polymerase synthesizes a new DNA strand. Buffers are required for optimal activity and stability of the DNA polymerase enzyme. Magnesium ions are necessary for maximum Taq polymerase activity.
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