New Protein Molecule Design MCQ

Test your knowledge with important New Protein Molecule Design MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30+ more New Protein Molecule Design MCQs on Bissoy. Bissoy App

• In the case of a polyvalent display, the proteins can be cloned directly into a viral vector as a fusion to a capsid protein.
  ক

  False

  খ

  True
• In the case of polyvalent-display, all gp III are conjugated with peptide and this results in occasional loss of infectivity.
  ক

  False

  খ

  True
• Which of the following is used to create a large library of mutants for a given protein gene?
  ক

  Protein assay

  খ

  Phage display

  গ

  X-ray crystallography

  ঘ

  Error-prone PCR
• In the case of a monovalent display, the protein fusion can be constructed in a phagemid vector that carries a copy of the viral capsid gene.
  ক

  False

  খ

  True
• Which of the following proteins are involved in initiating phage assembly and maintaining the stability of the viral particle?
  ক

  gp III and gp VIII

  খ

  gp VIII and gp II

  গ

  gp VI and gp IV

  ঘ

  gp VII and gp IX
• Which of the following is not true for error-prone PCR?
  ক

  It uses Taq polymerase which lacks proofreading ability

  খ

  Occurs under imperfect PCR conditions

  গ

  Involves annealing incompatible base-pairs to each other

  ঘ

  It is not a mutagenesis method
• Which of the following is the major coat protein of M13?
  ক

  gp VI

  খ

  gp V

  গ

  gp IV

  ঘ

  gp VIII
• M13 is a filamentous phage and it contains a circular single-stranded DNA genome.
  ক

  False

  খ

  True
• Which of the following bacteriophage is used in phage-display technology?
  ক

  T4

  খ

  λ

  গ

  T2

  ঘ

  M13
• In the case of monovalent display, the valency of display depends on the ratio of phage containing phagemid vector and helper phage at the time of infection.
  ক

  False

  খ

  True
• Who pioneered phage-display technology?
  ক

  Kary Mullis

  খ

  Charles Chamberland

  গ

  Robert brown

  ঘ

  George Smith
• Which of the following is not a method of directed evolution?
  ক

  Phage display

  খ

  DNA shuffling

  গ

  Error-prone PCR

  ঘ

  Natural selection
• Which step is performed after the mutation of genes in directed evolution?
  ক

  X-ray crystallography

  খ

  Protein assay

  গ

  Protein screening

  ঘ

  Expression of mutant genes
• The starting point of both rational and irrational protein design is a wild-type protein.
  ক

  False

  খ

  True
• Only one cycle of directed evolution cycle is sufficient to obtain the desired protein molecule.
  ক

  True

  খ

  False
• If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification?
  ক

  10,420

  খ

  5,120

  গ

  20,480

  ঘ

  10,240
• What is the temperature of the reaction mixture in the extension/elongation step of PCR?
  ক

  90-94°C

  খ

  50-65°C

  গ

  100-110°C

  ঘ

  68-72°C
• If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification?
  ক

  23,258,369

  খ

  5,456,987

  গ

  7,256,458

  ঘ

  8,388,608
• Which of the following cannot produce errors in error-prone PCR?
  ক

  High concentration of dATPs in the reaction mixture

  খ

  High concentration of Mg+2

  গ

  High concentration of dTTPs in the reaction mixture

  ঘ

  Taq polymerase with proof-reading ability
• What is the temperature of the reaction mixture in the second (annealing) step of PCR?
  ক

  100-110°C

  খ

  90-94°C

  গ

  70-72°C

  ঘ

  50-65°C
• In error-prone PCR the Taq polymerase anneals incompatible base-pairs to each other during amplification.
  ক

  False

  খ

  True
• Which of the following technique allows, the initiation of DNA amplification, starting with tiny amounts of the parent molecule, and produces considerable amounts of mutated genes?
  ক

  Southern Blotting

  খ

  Western Blotting

  গ

  PCR

  ঘ

  Error-prone PCR
• What is the temperature of the reaction mixture in the initiation step of PCR?
  ক

  70-72°C

  খ

  100-110°C

  গ

  68-70°C

  ঘ

  94-96°C
• PCR machine is also known as thermo-cycler.
  ক

  False

  খ

  True
• Which of the following is not supposed to be present in the PCR reaction mixture solution?
  ক

  Deoxynucleotide triphosphates (dNTPs)

  খ

  Magnesium ions

  গ

  Buffer

  ঘ

  Calcium ions
• Which of the following component is not required in a basic PCR?
  ক

  DNA template

  খ

  Two primers

  গ

  Thermostable DNA polymerase

  ঘ

  Initiation and Elongation Factors
• Which of the following technique is used to amplify a precise fragment of DNA from a complex mixture of starting material called template DNA?
  ক

  Northern Blotting

  খ

  Southern Blotting

  গ

  Western Blotting

  ঘ

  PCR
• Who discovered PCR?
  ক

  Charles Chamberland

  খ

  Linus Pauling

  গ

  Robert brown

  ঘ

  Kary Mullis
• Directed evolution screens for new enzyme activities by constructing a library of different enzymes derived from the same original protein.
  ক

  False

  খ

  True
• Which of the following technique is used to alter the function of an enzyme without the need for exhaustive structural and functional information?
  ক

  Slow evolution

  খ

  Indirect evolution

  গ

  Rational protein design

  ঘ

  Directed evolution