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Tomatoes contain lycopene (λmax = 444, 470 and 502 nm) and β-carotene (λmax = 442 and 472 nm). Which technique could be used for the analysis of lycopene and β-carotene in tomatoes, after suitable sample preparation? 

Tomatoes contain lycopene (λmax = 444, 470 and 502 nm) and β-carotene (λmax = 442 and 472 nm). Which technique could be used for the analysis of lycopene and β-carotene in tomatoes, after suitable sample preparation?  Correct Answer HPLC with UV-VIS detection

HPLC with UV-VIS detection

  • HPLC with UV-VIS detection is the technique that could be used for the analysis of lycopene and β-carotene in tomatoes, after suitable sample preparation.
  • Its detectors operate by passing visible and ultraviolet light through a sample in an exceedingly flow cell, then measure the absorption of the various wavelengths that pass through the cell. The number of light absorbed provides data on the properties of the sample of interest.
  • The major carotenoids within the red carrot and tomato paste are lycopene and β-carotene however different carotenoids, like α-carotene and little amounts of xanthophylls, might additionally interfere with the absorption of lycopene.

Additional Information

1. Atomic absorption spectroscopy (AAS) and atomic emission spectroscopy (AES) may be a spectroanalytical procedure for the quantitative determination of chemical components exploitation the absorption of optical radiation (light) by free atoms within the aerosolized state. Atomic absorption spectroscopy relies on the absorption of light by free metallic ions.

2. HPLC with UV detection

HPLC UV detectors are used with high-performance liquid chromatography to detect and identify analytes within the sample. A UV visible HPLC detector uses light to investigate samples. By activity the sample's absorption of light at completely different wavelengths, the analyte may be identified.

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