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This answer is different depending on the instrument being played. However, the A at the top of the bass clef staff is 220Hz. The A in the bottom space of the staff is 110Hz, and therefore sounds lower. If playing a cello, the higher A (220Hz) is on the A strings, while the lower A (110Hz) is first finger on the G string. On an electric bass, the higher A is on the second fret of the G string (fourth string), while the lower A is zero fingers on the A string (second string).

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