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In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells.
(i) Have origin of replication(Ori) (ii) a selectable marker (iii) at least one recognition site.
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For producing a recombinant protein (for therapeutic purpose) in large scale, which vector would you choose
High-copy-number, because higher the copy number of vector plasmid, higher the copy number of gene and consequently, the protein coded by the gene is produced in high amount.
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A wine maker and a molecular biologist who has developed a recombinant vaccine, both claim themselves to be biotechnologist.
Both. As biotechnology is a very wide area which deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organism to produce products and...
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You have created a recombinant DNA molecule by ligating a gene to a plasmid vector
The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which...
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The restriction enzymes that are used in construction of recombinant DNA molecule are endonuclease
If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky...
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Define the terms Antigen and Antibody. Name any two diagnostic kits based upon them.
An antigen is a foreign substance that elicits the formation of an antibody. Antibody is a protein that is synthesised in response to an antigen. Antigen and antibody show high degree of specificity in binding each...
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ELISA technique is based on the principles of antigen and antibody interaction.
Yes. One can use antibody against the enzyme (that is responsible for the metabolism of phenylalanine) to develop ELISA based diagnostic technique. The patient where the enzyme protein is absent...
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What would happen when you grow a recombinant in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing.
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids...
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Define the terms Antigen and Antibody
An antigen is a foreign substance that elicits the formation of an antibody. Antibody is a protein that is synthesised in response to an antigen. Antigen and antibody show high...
1 Answers 1 views
ELISA technique is based on the principles of antigen and antibody interaction. Can this technique be used in the molecular diagnosis
Yes. One can use antibody against the enzyme (that is, responsible for the metabolism of phenylalanine) to develop ELISA based diagnostic technique. The patient where the enzyme protein is absent...
1 Answers 1 views