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In enzymology, a tRNA sulfurtransferase is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are L-cysteine and 'activated' tRNA, whereas its two products are L-serine and tRNA containing a thionucleotide.

This enzyme belongs to the family of transferases, specifically the sulfurtransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is L-cysteine:tRNA sulfurtransferase. Other names in common use include transfer ribonucleate sulfurtransferase, RNA sulfurtransferase, ribonucleate sulfurtransferase, transfer RNA sulfurtransferase, and transfer RNA thiolase.

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