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Why is the number of colonies on the tetracycline agar for the treated bacteria limited to instead of showing numerous colonies?

Why is the number of colonies on the tetracycline agar for the treated bacteria limited to instead of showing numerous colonies? Correct Answer Not all treated bacteria absorbed the recombinant plasmid.

D. Not all of the treated bacteria were successfully transformed. Some of the bacteria did not absorb the plasmid, while others may have absorbed the plasmid but were unable to express the antibiotic-resistant gene.

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