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Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Which of the following statements is/are correct regarding PCR (Polymerase Chain Reaction)? 1. In this reaction, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. 2. Real-time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus (COVID-19).  Select the correct answer using the code given below.
The Taq DNA polymerase is DNA polymerase _________ enzyme from the bacterium Thermus aquaticus.
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________
How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules?
Which of the following enzyme catalyses the direct transfer and incorporation of O2 into a substrate molecule is
Which enzyme catalyses the process of transamination?
The enzyme which catalyses the conversion of proteins to amino acids is ______
The enzyme that catalyses carbon dioxide fixation in C4 plants is
The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase