Animal Biotechnology MCQ

Test your knowledge with important Animal Biotechnology MCQ and their applications. These MCQs are beneficial for competitive exams too. Explore 30+ more Animal Biotechnology MCQs on Bissoy. Bissoy App

• Who invented that the enzyme DNA polymerase catalyses a 5' → 3' and 3' → 5' exonucleolytic degradation of DNA
  ক

  Nirenberg

  খ

  Kornberg

  গ

  Mathaei

  ঘ

  Khorana
• The ABO blood groups of humans are determined by three alleles. How many genotypes are possible for these phenotypes
  ক

  3

  খ

  6

  গ

  9

  ঘ

  27
• Which of the following statements are correct about the function of fetal bovine serum in animal cell culture?<br>P. It stimulates cell growth.<br>Q. It enhances cell attachment.<br>R. It provides hormones and minerals.<br>S. It maintains pH at 7.4.
  ক

  P and Q only

  খ

  P and S only

  গ

  P, Q and R only

  ঘ

  P, Q, R and S
• The product commercially produced by animal cell culture is
  ক

  Insulin

  খ

  Tissue plasminogen activator

  গ

  Interferon

  ঘ

  Hepatitis B vaccine
• All of the following are produced by animal cells in culture and help the cells adhere to the culture dish except
  ক

  Glycoproteins

  খ

  Collagen

  গ

  Phospholipase A

  ঘ

  Hyaluronic acid
• Gene therapy that is hereditary relates to
  ক

  Somatic cells

  খ

  Germline cells

  গ

  Cancer cells

  ঘ

  Udder cells
• Which one of the following is NOT used for the measurement of cell viability in animal cell culture?
  ক

  Trypan blue dye exclusion

  খ

  Tetrazolium (MTT) assay

  গ

  LDH activity in the culture medium

  ঘ

  Coulter counter
• Receptor R is over expressed in CHO cells and analyzed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm. the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)
  ক

  10⁴

  খ

  10⁵

  গ

  10⁶

  ঘ

  10⁷
• r-HuEPO is
  ক

  Human genome

  খ

  Hybridoma technology

  গ

  Recombinant Erythropoietin

  ঘ

  None of these
• A bacterial culture contains 108 cells/ml in the beginning. Two hours later, it was found to contain 10¹² cells/ml. Calculate the doubling time.
  ক

  0.30 hr

  খ

  0.15 hr

  গ

  0.24 hr

  ঘ

  0.50 hr
• A hybrid derived from the fusion of a myeloma cell (HPRT⁻) with an antibody secreting B-lymphocyte (HPRT⁺) can be selected to produce monoclonal antibody by growing in a medium containing
  ক

  Thiamine, Hypoxanthine, Aminopterin

  খ

  Thymidine, Histidine, Aminopterin

  গ

  Uridine, Hypoxanthine, Aminopterin

  ঘ

  Thymidine, Hypoxanthine, Aminopterin
• For the growth of T-cell, the growth factor needed would be
  ক

  Epidermal growth factor

  খ

  Interleukin-2

  গ

  Fibroblast growth factor

  ঘ

  TNF-α
• Match the following reporter gene with their source<br>
  ক

  <table class="table-style-1"> <tr> <td><strong>Column-I</strong></td> <td><strong>Column-II</strong></td> </tr> <tr> <td>A. β-Galactosidase</td> <td>1. Firefly</td> </tr> <tr> <td>B. Lux</td> <td>2. E. coli</td> </tr> <tr> <td>C. TK</td> <td>3. Herpers simplex virus</td> </tr> <tr> <td>D. Blasticidin deaminase (bed)</td> <td>4. Aspergillus terrcus</td> </tr></table> A-3, B-2, C-1, D-4

  খ

  A-2, B-1, C-3, D-4

  গ

  A-3, B-1, C-2, D-4

  ঘ

  A-2, B-4, C-3, D-1
• Hybridoma technology is used to produce
  ক

  Monoclonal antibodies

  খ

  Polyclonal antibodies

  গ

  Both monoclonal and polyclonal antibodies

  ঘ

  B cells
• Methotrexate (MTX) resistant cell lines developed through selection show
  ক

  Increased MYX uptake

  খ

  Reduced production of DHFR

  গ

  Changed DHFR with reduced affinity for MTX

  ঘ

  None of the above
• Detection of which hormone is the commonly used test for pregnancy in humans?
  ক

  LH

  খ

  FSH

  গ

  Chorionic gonadotropin

  ঘ

  Estrogen
• Gene targeting is done on an
  ক

  Sperm cell

  খ

  Egg cell

  গ

  Fertilized ovum

  ঘ

  Early embryonic cell
• Positional cloning approach exploits information
  ক

  On the location of the gene in the genome

  খ

  On the status of its expression

  গ

  About the position of promoter relative to MCS

  ঘ

  About the position of the restriction sites
• When electroporation is used for introducing DNA into mammalian cell<br>P. A carrier for DNA is not required.<br>Q. The lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites.<br>R. The viability of the cell becomes approximately zero percent.<br>S. The first step involves absorption of DNA on the cell membrane.
  ক

  P, Q

  খ

  Q, R

  গ

  P, S

  ঘ

  Q, S
• Which of the following DNA Pol has proofreading property in animal cells?
  ক

  DNA Pol α

  খ

  DNA Pol δ

  গ

  DNA Pol β

  ঘ

  All of the above
• Choose the correct combination of True (T) and False (F) statements about microcarriers used in animal cell culture.<br>P. Higher cell densities can be achieved using microcarriers.<br>Q. Microcarriers increase the surface area for cell growth.<br>R. Microcarriers are used for both anchorage- and non-anchorage-dependent cells.<br>S. Absence of surface charge on microcarriers enhances attachment of cells.
  ক

  P-T; Q-F; R-T and S-F

  খ

  P-T; Q-T; R-F and S-F

  গ

  P-F; Q-F; R-T and S-T

  ঘ

  P-F; Q-T; R-F and S-T
• Production of monoclonal antibodies by hybridoma technology requires
  ক

  Splenocytes

  খ

  Osteocytes

  গ

  Hepatocytes

  ঘ

  Thymocytes
• Which of the following statement is not true for Xanthine-guanine phosphoribosyl transferase (XGPRT)?
  ক

  It is an enzyme of endogeneous pathway

  খ

  Derived from E. coli it serve as reporter gene

  গ

  Convert hypoxanthine into IMP

  ঘ

  Convert guanine into GMP
• The genes which promote cancerous growth are called as
  ক

  Oncogenes

  খ

  Tumour suppressor genes

  গ

  Both A and B

  ঘ

  None of the above
• Transgenic pharming involves using . . . . . . . . to produce recombinant proteins in . . . . . . . .
  ক

  Cows; Milk

  খ

  Phage; Bacteria

  গ

  Bacteria; Culture media

  ঘ

  Fungi; Culture media
• Nitrogen fixing genes are called
  ক

  Nif' genes

  খ

  Plasmid genes

  গ

  Leg genes

  ঘ

  Cos genes
• The culture fluids of 1000 to 5000 colonies of hybridoma are screened for monoclonal antibody by<br>P. Western blot analysis.<br>Q. Antigen capture analysis.<br>R. Northern blot analysis.<br>S. Antibody capture analysis.<br>Choose the correct pair from the following
  ক

  P, Q

  খ

  Q, R

  গ

  R, S

  ঘ

  Q, S
• To promote attachment and spreading of anchorage-dependent animal cells the surface of the culture vessel needs to be coated with
  ক

  Trypsin

  খ

  Collagen

  গ

  Pronase

  ঘ

  Polyglycol
• When the value of selection coefficient is unity (S = 1), there would be
  ক

  No survival

  খ

  10% survival

  গ

  50% survival

  ঘ

  75% survival
• Which of the following has been produced commercially from mammalian cell cultures?
  ক

  Plasminogen activator

  খ

  Antibacterial antibiotics

  গ

  Insulin

  ঘ

  Renin